Rodent Sex

Number for Behavior

Total for low range

∑ = 310

Total for high range

∑ = 610

Behavior

Methodology

Observe Before Perturb?

Comprehensive Data Table

Use the filters below, to quickly search the data table. To add your own data to the table, simply click “+ New Entry” where designated below. Refer to the “Need Help?” link, for further assistance.

To quickly browse through the table columns, without excessive vertical scrolling, use your < or > arrows.

Need help?

wdt_ID Target region Animal Model Strain Sex n for behavior – low range n for behavior – high range Behavior Methodology Virus Stimulation pattern or CNO dose Impact on anxiety behaviors Impact on fear behaviors Impact on depressive behaviors Reference Lab name PMID (PubMed ID) Other behaviors run? Post-hoc confirmation of injections Observe before perturb?
1 mPFC Mice C57 Male 6 10 Chronic social defeat Optogenetics HSV-ChR2-mCherry, in HSVPrpUC,using the ChR2-mCherry DNA construct or mCherry alone (control) ChR2 @ 40 ms 100 Hz light pulses every 3 seconds over the duration of each behavioral test No change Open field test Photostimulation of mPFC reverses depression-like symptoms in mice susceptible to social defeat Covington et al., 2010 Eric J. Nestler 21.123.555 Yes, IEG expression run, p Yes
2 Social interaction
3 Sucrose preference test
4 LA pyramidal neurons Rats Sprague-Dawley Male 8 8 Fear conditioning Optogenetics AAV-CaMKII-GFP, pAAV-MCS, pAAV-CaMKII-ChR2/EYFP CaMKII-ChR2 in LA @ 20-Hz, 10 ms pulse stimulation Pairing an auditory cue with optical stimulation (no shock US) increased freezing/produced fear conditioning Johansen et al., 2010 Joseph E. LeDoux 20.615.999 Yes, noted was confirmed Yes
5 dCA1 Mice C57 Unknown 4 6 Fear conditioning Optogenetics AAV-CaMKIIa-eNpHR3.0-EYFP, using BamHI and EcoRI restriction sites, AAV CaMKIIa::eNpHR3.0 and Lenti CaMKIIa::eNpHR3.1 CaMKIIα-eNpHR3.1 @ continuous light No difference in Open field test Photoinhibition during training prevents contextual fear memory/no effect recall. Photoinhibition during recall prevents fear recall Goshen et al., 2011 Karl Deisseroth 22.019.004 Yes, when validated optogenetic activation found a significant increase in ACC neuronal activity in control mice, which was abolished in mice expressing eNpHR3.0 in ACC No
6 Open field test
7 ACC Mice C57 Unknown 4 6 Fear conditioning Optogenetics AAV-CaMKIIa-eNpHR3.0-EYFP, using BamHI and EcoRI restriction sites, AAV CaMKIIa::eNpHR3.0 and Lenti CaMKIIa::eNpHR3.1 CaMKIIα-eNpHR3.1 @ continuous light No difference in Open field test Photoinhibition prevents long term fear recall (one month later) Goshen et al., 2011 Karl Deisseroth 22.019.004 Yes, when validated optogenetic activation found a significant increase in ACC neuronal activity in control mice, which was abolished in mice expressing eNpHR3.0 in ACC No
8 Open field test
9 DG Mice c-fos-tTA, TetTag, C57BL/6J Unknown 5 12 Fear conditioning Optogenetics AAV9-TRE-ChR2-EYFP Optogenetic reactivation of neurons activated during fear conditioning with c-fos-tTA transgenic mice + TRE-ChR2 + removal of DOX during training @ 20Hz trains during light-on (3 min off-on-off-on) Optogenetic reactivation induces freezing behavior Liu et al., 2012 Susumu Tonegawa 22.441.246 Yes, histologically verified Yes
10 mPFC Rats Long-Evans Male 8 16 Forced swim test Optogenetics pAAV-CaMKIIα∷hChR2(H134R)-eYFP, pAAV-CaMKIIα∷eYFP, pAAVCaMKIIα∷eNpHR3.0-eYFP, and pAAV-hSyn∷hChR2(H134R)-eYFP into mPFC or DRN CaMKIIα-ChR2 with fiber over DRN or LhB @ pulse trains with 5 ms long 473 nm light pulses at 20 Hz No effect in Open field test No effect in forced swim test Warden et al., 2012 Karl Deisseroth 23.160.494 Yes, IHC and optical microscopic confirmation, staining for DAPI and either rabbit anti-5-HT or rabbit anti-GABA No
Target region Animal Model Strain Sex n for behavior – low range n for behavior – high range Behavior Methodology Virus Stimulation pattern or CNO dose Impact on anxiety behaviors Impact on fear behaviors Impact on depressive behaviors Reference Lab name PMID (PubMed ID) Other behaviors run? Post-hoc confirmation of injections Observe before perturb?
Close Bitnami banner
Bitnami