Rodent Sex
Number for Behavior
Total for low range
∑ =
310
Total for high range
∑ =
610
Behavior
Methodology
Observe Before Perturb?
Comprehensive Data Table
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wdt_ID | Target region | Animal Model | Strain | Sex | n for behavior – low range | n for behavior – high range | Behavior | Methodology | Virus | Stimulation pattern or CNO dose | Impact on anxiety behaviors | Impact on fear behaviors | Impact on depressive behaviors | Reference | Lab name | PMID (PubMed ID) | Other behaviors run? | Post-hoc confirmation of injections | Observe before perturb? |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
1 | mPFC | Mice | C57 | Male | 6 | 10 | Chronic social defeat | Optogenetics | HSV-ChR2-mCherry, in HSVPrpUC,using the ChR2-mCherry DNA construct or mCherry alone (control) | ChR2 @ 40 ms 100 Hz light pulses every 3 seconds over the duration of each behavioral test | No change Open field test | Photostimulation of mPFC reverses depression-like symptoms in mice susceptible to social defeat | Covington et al., 2010 | Eric J. Nestler | 21.123.555 | Yes, IEG expression run, p | Yes | ||
2 | Social interaction | ||||||||||||||||||
3 | Sucrose preference test | ||||||||||||||||||
4 | LA pyramidal neurons | Rats | Sprague-Dawley | Male | 8 | 8 | Fear conditioning | Optogenetics | AAV-CaMKII-GFP, pAAV-MCS, pAAV-CaMKII-ChR2/EYFP | CaMKII-ChR2 in LA @ 20-Hz, 10 ms pulse stimulation | Pairing an auditory cue with optical stimulation (no shock US) increased freezing/produced fear conditioning | Johansen et al., 2010 | Joseph E. LeDoux | 20.615.999 | Yes, noted was confirmed | Yes | |||
5 | dCA1 | Mice | C57 | Unknown | 4 | 6 | Fear conditioning | Optogenetics | AAV-CaMKIIa-eNpHR3.0-EYFP, using BamHI and EcoRI restriction sites, AAV CaMKIIa::eNpHR3.0 and Lenti CaMKIIa::eNpHR3.1 | CaMKIIα-eNpHR3.1 @ continuous light | No difference in Open field test | Photoinhibition during training prevents contextual fear memory/no effect recall. Photoinhibition during recall prevents fear recall | Goshen et al., 2011 | Karl Deisseroth | 22.019.004 | Yes, when validated optogenetic activation found a significant increase in ACC neuronal activity in control mice, which was abolished in mice expressing eNpHR3.0 in ACC | No | ||
6 | Open field test | ||||||||||||||||||
7 | ACC | Mice | C57 | Unknown | 4 | 6 | Fear conditioning | Optogenetics | AAV-CaMKIIa-eNpHR3.0-EYFP, using BamHI and EcoRI restriction sites, AAV CaMKIIa::eNpHR3.0 and Lenti CaMKIIa::eNpHR3.1 | CaMKIIα-eNpHR3.1 @ continuous light | No difference in Open field test | Photoinhibition prevents long term fear recall (one month later) | Goshen et al., 2011 | Karl Deisseroth | 22.019.004 | Yes, when validated optogenetic activation found a significant increase in ACC neuronal activity in control mice, which was abolished in mice expressing eNpHR3.0 in ACC | No | ||
8 | Open field test | ||||||||||||||||||
9 | DG | Mice | c-fos-tTA, TetTag, C57BL/6J | Unknown | 5 | 12 | Fear conditioning | Optogenetics | AAV9-TRE-ChR2-EYFP | Optogenetic reactivation of neurons activated during fear conditioning with c-fos-tTA transgenic mice + TRE-ChR2 + removal of DOX during training @ 20Hz trains during light-on (3 min off-on-off-on) | Optogenetic reactivation induces freezing behavior | Liu et al., 2012 | Susumu Tonegawa | 22.441.246 | Yes, histologically verified | Yes | |||
10 | mPFC | Rats | Long-Evans | Male | 8 | 16 | Forced swim test | Optogenetics | pAAV-CaMKIIα∷hChR2(H134R)-eYFP, pAAV-CaMKIIα∷eYFP, pAAVCaMKIIα∷eNpHR3.0-eYFP, and pAAV-hSyn∷hChR2(H134R)-eYFP into mPFC or DRN | CaMKIIα-ChR2 with fiber over DRN or LhB @ pulse trains with 5 ms long 473 nm light pulses at 20 Hz | No effect in Open field test | No effect in forced swim test | Warden et al., 2012 | Karl Deisseroth | 23.160.494 | Yes, IHC and optical microscopic confirmation, staining for DAPI and either rabbit anti-5-HT or rabbit anti-GABA | No |